Controlled expansion and differentiation of mesenchymal stem cells in a microcarrier based stirred bioreactor
نویسندگان
چکیده
Introduction Cell based therapy requires great numbers of cells in a functional state permitting their in vivo implantation for the restoration of tissue homeostasis. Three main parameters are believed to be essential for such a purpose: an appropriate cell population, a suitable scaffold and appropriate physical / biochemical factors enabling proper expansion and in vitro cell differentiation. In recent years, mesenchymal stem cells (MSCs) have been attracting a lot of interest in this field, because of their differentiation potential and their trophic factor secretion abilities. The aim of this work is to perform a rational analysis of key factors involved in the efficient proliferation and differentiation of MSCs, in the context of a stirred microcarrier (MC)-based bioreactor.
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